Excitation 372nm, with an emission at 456nm giving a blue colour. Cell morphology was first recorded under light microscopy (Nikon Eclipse TS100, Nikon, Sydney, Australia) and then the fluorescence images were captured using a Nikon Eclipse DAPI staining (blue) of chromosomes, multiplexed with FISH probes (green and red). Dilute DAPI stock solution to a concentration between 1.60-0.400 µg/ml in PBS and incubate for 15 min at room temperature in the dark before analyzing cells on flow cytometer. 2. You can now find all Web Scientific products on our parent company's site. Dilute the 300 µM DAPI intermediate dilution 1:1,000 in PBS as needed to make a 300 nM DAPI stain solution. chloroform $ 40.40 $ 25.45. g 418 disulfate salt solution sterile $ 553.00 $ 541.94. sku: g8168-100ml + sigma. + sigma. Clinisciences > > > DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A … Manufacturer: MilliporeSigma™ 5.08741.0001 Protect from light. SKU. There was an error retrieving your Wish Lists. Add to Cart. 5087410001 DescriptionDAPI (4-6 Diamino-2-Phenylindole) is a nucleic acid stain used in COMET assays for detecting nuclear DNA damage in Geno-toxicity testing. The In lyophilized form, the chemical is stable for 24 months. Compare Products: Select up to 4 products. DAPI- 5mg/ml- 1ml quantity Cell p This product is supplied as a 5mg/ml aqueous solution in a dropper bottle. Search results for D9542-5mg at Sigma-Aldrich. Add 2.1 µL of the 14.3 mM DAPI stock solution to 100 µL PBS to make a 300 µM DAPI intermediate dilution: 3. MilliporeSigma™ Calbiochem™ DAPI Stain Serves as sterile-filtered solution of DAPI in water. +39 055 722311 WhatsApp (messages only) +39 3428498479 email: customercare@sigmagi.it To calculate the overall star rating and percentage breakdown by star, we don’t use a simple average. DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining, and flow cytometry. Radway Green Business Centre | Biochem/physiol Actions . Frequently Asked Questions are available for this Product. DAPI dilactate C16H15N5 • 2 C3H6O3 457.48 N/A Introduction DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. Unable to add item to List. After incubation overnight, labeled cells Its blue fluorescence stands out in vivid contrast to green, yellow or red fluorescent probes of other structures. Manufacturer: MilliporeSigma™ 5087410001 Catalog No. Frequently Asked Questions are available for this Product. By continuing, you confirm that you accept our policy statement. Probe coating. Note:Do not use any buffers. Your recently viewed items and featured recommendations, Select the department you want to search in. Then the cell culture media contain-ing both Feridex-PLL and DAPI were kept at 378C in a 95% air:5% CO 2 atmosphere. 0.75mg/ml. O) or dimethylformamide (DMF). Preparing the DAPI Stock SolutionTo make a 5 mg/mL DAPI stock solution (14.3 mM for the dihydrochloride or 10.9 mM for the dilactate), dissolve the contents of one vial (10 mg) in 2 mL of deionized water (dH. sale! It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. ... sigma. 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